qPCR Transcriptome Fingerprinting Core This core facility provides free assays for up to 30 target transcripts in triplicate (100 total samples) to Centers funded under the NIAID program: “Cooperative Centers for Translational Research on Human Immunology and Biodefense.”  Additional assays are offered at cost.  See below for important information regarding the use of this core.  For additional information or questions, contact Esther Rhee via email at esther.rhee@mssm.edu or by phone at 212-241-1710.  INFORMATION  §                For a list of our current human and mouse validated assays, click here. §                If you are interested in the Core’s validated assays, please fill out the linked RNA spreadsheet giving sample information and transcript selection for assay.  The form may be returned to Esther Rhee via email.  Click here for RNA spreadsheet (excel document). §                The core can also design up to 10 new assays for each Center.  Most of our T cell panel is still in beta testing.  For a transcript that is not listed, please fill out the linked form and return to Esther Rhee via email.  Click here for Design Request (word document).  §                Assays on existing reactions will be completed and results emailed as quickly as possible, normally within 5 working days.  New assays will be established as quickly as possible, usually requiring approximately 2 weeks.  §                All data is confidential, publication quality and provided with full data for publication (protocol used, primer sequence, etc.).  Data is provided in raw and estimated copy number using the Global Normalization Algorithm.  Click here for a copy (adobe document).   SAMPLE PREPARATION  Samples should be prepared as described below: 1.      We prefer at least 2 micrograms of total RNA purified using Trizol or standard matrix kits (Stratagene, etc.).  We will work with no less than 1 microgram. 2.      Should be dissolved in DEPC water or Elution Buffer 3.      Preferably at a concentration of 200-900 ng/microliter (but not necessary) 4.      Each sample should be in a different 1.5 mL eppendorf tube, marked 1-N (N being the number of tubes) 5.      Fill out RNA form and all the information required on it. 6.      Please send on dry ice to: Esther Rhee/Sealfon Laboratory Mount Sinai School of Medicine Annenberg 14-70 Neurology, Box 1137 One Gustave L. Levy Place                          New York, NY 10029       VALIDATED ASSAYS   (back to top)